MORPHOFUNCTIONAL CHANGES IN THE LIVER OF THE OFFSPRING OF RATS WITH INDUCED CHOLESTASIS
Abstract
Background. The influence of cholestasis of pregnant women on the development of the liver in their offspring is not well studied.
Objective. The aim of the study was to determine the effect of the experimental cholestasis of the mother on the morphofunctional state of the liver of 2 and 15-day progeny and the possibility of correcting the disorders with ursodeoxycholic acid.
Materials and methods. In the research the material from 30 outbred white rats, histological, histochemical, morphometric and statistical methods of investigation were used.
Results. It was established that the females of the experimental group undergo prolongation of pregnancy; the rats are born weak and less healthy. On the 2nd day of postnatal development in the experimental group "Cholestasis" an increase in the glycogen content is observed, the activity of succinate dehydrogenase is reduced, and NADH dehydrogenase is increased. Under the influence of mother’s cholestasis, sinusoidal capillaries dilate in the liver of the 15-day progeny, the sizes of hepatocytes are reduced, the activity of NADH dehydrogenase decreases, the activity of lactate dehydrogenase and acid phosphatase increases in the cytoplasm.
Conclusion. Ursodeoxycholic acid partially restores the structure and metabolism of hepatocytes throughout the study period.
References
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